LY6 抗体 (APC)
The APC-conjugated 大鼠 单克隆 anti-LY6 antibody (Clone D7) (ABIN7840913) specifically detects LY6 in FACS 和 IF.
The antibody is reactive with 小鼠 samples.
产品编号 ABIN7840913
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for LY6 抗体 (APC) (ABIN7840913)
抗原
LY6
(Lymphocyte Antigen 6 Complex (LY6))
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
This LY6 antibody is conjugated to APC
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
D7
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原理
- Rat monoclonal antibody APC labled to Ly-6A/E
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特异性
- Recognizes mouse Ly-6A/E
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- LY6 (Lymphocyte Antigen 6 Complex (LY6))
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别名
- Ly6
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背景
- Ly6, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly67, Sca-2, Tsa-1, Lymphocyte antigen 6E, Ly-6E, Stem cell antigen 2, Thymic shared antigen 1, TSA-1
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基因ID
- 110454
抗原
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