IL-5 抗体
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北京 101111
Quick Overview for IL-5 抗体 (ABIN7840044)
抗原
See all IL-5 (IL5) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Rat monoclonal antibody to IL-5
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特异性
- Recognizes human/mouse IL-5
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Partially-purified T cell clone supernatant
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anti-Interleukin 5 (IL5) (AA 20-134) antibody
IL5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 21-70) antibodyIL5 适用: 人, 小鼠, 大鼠, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 56-132) antibodyIL5 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 山羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 人 ELISA, D, ELISA (Capture) 宿主: 大鼠 Monoclonal JES1-39D10 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 小鼠 ELISA 宿主: 大鼠 Monoclonal TRFK5 unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- IL5
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背景
- Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF
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基因ID
- 3567, 16191
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UniProt
- P05113
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原
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