IL12B 抗体
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北京 101111
Quick Overview for IL12B 抗体 (ABIN7840010)
抗原
See all IL12B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody to IL-12 p40
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特异性
- Recognizes human IL-12 p40
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Recombinant human IL-12 (p40)
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anti-Interleukin 12 beta (IL12B) (AA 201-300) antibody
IL12B 适用: 人, 小鼠 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) (AA 30-320) antibodyIL12B 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 12 beta (IL12B) (AA 229-328) antibodyIL12B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H6 unconjugated
anti-Interleukin 12 beta (IL12B) (Internal Region) antibodyVerified IL12B 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) (Internal Region) antibodyVerified IL12B 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) (AA 23-328) antibodyIL12B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) (AA 23-327) antibodyIL12B 适用: 绵羊 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) (AA 35-302) antibodyIL12B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 beta (IL12B) antibodyIL12B 适用: 人, Pig ELISA, IHC (fro) 宿主: 小鼠 Monoclonal I-1A4 unconjugated
anti-Interleukin 12 beta (IL12B) (C-Term) antibodyIL12B 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- IL12B (Interleukin 12 beta (IL12B))
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别名
- IL12B
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背景
- NKSF2, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2
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基因ID
- 3593
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UniProt
- P29460
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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