CD9 抗体 (Biotin)
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北京 101111
Quick Overview for CD9 抗体 (Biotin) (ABIN7836672)
抗原
See all CD9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody Biotin labled to CD9
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特异性
- Recognizes human CD9
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD9.
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anti-CD9 (CD9) (AA 112-195) antibody
CD9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5A6D8 unconjugated
anti-CD9 (CD9) (AA 112-195) antibodyCD9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E8H1 unconjugated
anti-CD9 (CD9) (Internal Region) antibodyCD9 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CD9-1619 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal CD9-2343 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 5G6 unconjugated
anti-CD9 (CD9) (AA 101-200) antibodyCD9 适用: 人, 小鼠, Cow, 大鼠, Pig FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibody (Biotin)CD9 适用: 小鼠 FACS, WB, IP, ICC 宿主: 大鼠 Monoclonal EM-04 Biotin
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, WB, IHC (p), MS 宿主: 小鼠 Monoclonal MEM-61 unconjugated
anti-CD9 (CD9) (AA 115-145) antibodyCD9 适用: 人 FACS, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- CD9
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别名
- CD9
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背景
- MIC3, TSPAN29, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD antigen CD9
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基因ID
- 928
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UniProt
- P21926
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途径
- Response to Water Deprivation, Cell-Cell Junction Organization
抗原
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