CD8 alpha 抗体 (PerCP-Cy5.5)
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Quick Overview for CD8 alpha 抗体 (PerCP-Cy5.5) (ABIN7836612)
抗原
See all CD8 alpha (CD8A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody PerCP/Cy5.5 labled to CD8
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特异性
- Recognizes human CD8
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD8.
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anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 185-235) antibodyCD8A 适用: 人 FACS, IHC, WB, IF (p), mIF 宿主: 兔 Monoclonal BLR044F unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 FACS, IHC, IF 宿主: 小鼠 Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 FACS, WB, IF (p) 宿主: 兔 Monoclonal 23GB1420 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (p), ICC, IHC (fro) 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (p), ICC, IHC (fro), Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) (AA 28-196) antibodyCD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8A
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背景
- MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD antigen CD8b
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基因ID
- 925
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UniProt
- P01732
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途径
- TCR Signaling
抗原
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