CD52 抗体
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北京 101111
Quick Overview for CD52 抗体 (ABIN7836447)
抗原
See all CD52 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody to CD52
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特异性
- Recognizes human CD52
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Human tonsil
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anti-CD52 (CD52) antibody
CD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: Human Monoclonal Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) (AA 1-61) antibodyCD52 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal HI186 APC
anti-CD52 (CD52) antibodyCD52 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) antibodyCD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: 兔 Chimeric Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 IHC (f) 宿主: 兔 Monoclonal CD52-2276R unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 unconjugated
anti-CD52 (CD52) antibody (FITC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 FITC
anti-CD52 (CD52) antibody (PE)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 PE
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 APC
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- CD52
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别名
- CD52
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背景
- CDW52, HE5, CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5, CD52
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基因ID
- 1043
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UniProt
- P31358
抗原
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