ITGA4 抗体 (APC)
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Quick Overview for ITGA4 抗体 (APC) (ABIN7836415)
抗原
See all ITGA4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody APC labled to CD49d
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特异性
- Recognizes human CD49d
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD49d.
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anti-Integrin alpha 4 (ITGA4) (Internal Region) antibody
ITGA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 551-650) antibodyITGA4 适用: 人, 小鼠, 兔 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 833-977) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7E4C5 unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 376-558) antibodyITGA4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyKO Validated ITGA4 适用: 人 FACS, WB 宿主: 兔 Monoclonal 25GB2175 unconjugated Recombinant Antibody
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, EIA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal DATK32 unconjugated
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- ITGA4 (Integrin alpha 4 (ITGA4))
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别名
- ITGA4
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背景
- CD49D, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, CD antigen CD49d
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基因ID
- 3676
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UniProt
- P13612
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途径
- Integrin Complex
抗原
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