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Integrin Alpha2b 抗体 (APC)

The APC-conjugated 小鼠 单克隆 anti-Integrin Alpha2b antibody (Clone HIP8) (ABIN7836293) specifically detects Integrin Alpha2b in FACS 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN7836293
发货至: 中国
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Quick Overview for Integrin Alpha2b 抗体 (APC) (ABIN7836293)

抗原

See all Integrin Alpha2b (CD41) 抗体
Integrin Alpha2b (CD41)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Integrin Alpha2b antibody is conjugated to APC

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

HIP8
  • 原理

    Mouse monoclonal antibody APC labled to CD41

    特异性

    Recognizes human CD41

    纯化方法

    The antibody was purified by affinity chromatography.

    免疫原

    Native purified human CD41.
  • 应用备注

    Flow: 10 μL / assay

    实验流程

    1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Shipped and store at 4°C for one year. Do not freeze.
  • 抗原

    Integrin Alpha2b (CD41)

    别名

    ITGA2B

    背景

    GP2B, ITGAB, Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41

    基因ID

    3674

    UniProt

    P08514

    途径

    Integrin Complex
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