CD209b Antigen (CD209B) 抗体
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北京 101111
Quick Overview for CD209b Antigen (CD209B) 抗体 (ABIN7835948)
抗原
See all CD209b Antigen (CD209B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Armenian Hamster monoclonal antibody to CD209B
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特异性
- Recognizes mouse CD209B
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Partial mouse CD209B recombinant protein (74-325 aa)
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anti-CD209b Antigen (CD209B) (AA 51-150) antibody
CD209B 适用: 小鼠 WB, IF (cc), IF (p), IHC (fro), IHC (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD209b Antigen (CD209B) antibody (APC)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 APC
anti-CD209b Antigen (CD209B) antibody (PE)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 PE
anti-CD209b Antigen (CD209B) antibodyCD209B 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR9 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- CD209b Antigen (CD209B)
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别名
- CD209B
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背景
- CD209 antigen-like protein B, DC-SIGN-related protein 1, DC-SIGNR1, OtB7, CD209
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基因ID
- 69165
抗原
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