Fc Receptor, IgG, Low Affinity IV (FCGR4) 抗体
The Armenian Hamster 单克隆 anti-Fc Receptor, IgG, Low Affinity IV antibody is suitable to detect Fc Receptor, IgG, Low Affinity IV in samples from 小鼠. It has been validated for FACS 和 IF.
产品编号 ABIN7835824
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Fc Receptor, IgG, Low Affinity IV (FCGR4) 抗体 (ABIN7835824)
抗原
See all Fc Receptor, IgG, Low Affinity IV (FCGR4) 抗体
Fc Receptor, IgG, Low Affinity IV (FCGR4)
适用
小鼠
宿主
Armenian Hamster
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
9E9
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原理
- Armenian Hamster monoclonal antibody to CD16.2
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特异性
- Recognizes mouse CD16.2
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- FCgammaR4 -EC domain fusion with IgG1 Fc
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- Fc Receptor, IgG, Low Affinity IV (FCGR4)
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别名
- FCGR4
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背景
- Low affinity immunoglobulin gamma Fc region receptor IV, CD16-2, FcgammaRIV
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基因ID
- 246256
抗原
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