LexA 抗体
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北京 101111
Quick Overview for LexA 抗体 (ABIN7833406)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-LexA | LexA repressor
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交叉反应 （详细）
- Escherichia coli
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免疫原
- Full-size highly-purified recombinant LexA protein
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anti-LexA (AA 1-202) antibody
适用: E. coli ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-LexA (DNA Binding Domain) antibody适用: 化学剂 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1: 1000 - 1: 3000 (WB)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Serum. Contains 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- LexA
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背景
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Background: LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.Alternative names: exrA, spr, tsl, umuA
Additional information: This product can be used in:Studies of SOS regulation in E.coliDetection of bait constructs in yeast two-hybrid systemImmunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)Immunoprecipiation and ChIP
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分子量
- 22.3 | 23 kDa
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基因ID
- 948544
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UniProt
- P0A7C2
抗原
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