eGFP 抗体
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北京 101111
Quick Overview for eGFP 抗体 (ABIN7833234)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-EGFP | Enhanced Green Fluorescence Protein
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交叉反应 （详细）
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GFP, EGFP
Not reactive in: mCherry -
纯化方法
- Immunogen affinity purified
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免疫原
- Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli
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anti-Enhanced Green Fluorescent Protein (eGFP) (AA 1-238) antibody
eGFP 适用: 人, 大鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Enhanced Green Fluorescent Protein (eGFP) antibodyeGFP 适用: 标记 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enhanced Green Fluorescent Protein (eGFP) antibodyeGFP 适用: 标记 WB 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water, ultrapure water
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缓冲液
- Immunogen affinity purified in PBS pH 7.2-7.6, 0.1 % trehalose, 5 mM sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lypholized antibody at 4 °C. After reconstitution keep aliquots at -20°C for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened
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有效期
- 12 months
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- eGFP (Enhanced Green Fluorescent Protein (eGFP))
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别名
- EGFP
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背景
- EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
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分子量
- 27 kDa (for GFP without fusion partner)
抗原
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