Cyt F 抗体
The 兔 多克隆 anti-Cyt F antibody is suitable to detect Cyt F in samples from Chlamydomonas reinhardtii. It has been validated for WB.
产品编号 ABIN7833205
发货至:
中国
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Quick Overview for Cyt F 抗体 (ABIN7833205)
抗原
Cyt F
适用
Chlamydomonas reinhardtii
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB)
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原理
- Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)
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交叉反应 （详细）
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Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Euglena gracilis,Chlorella vulgaris,Synechocystis sp.,Nostoc sp.,Prochlorococcus marinus subsp. Pastoris,Synechococcus sp.,Prochlorococcus marinus,Synechococcus elongatus
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纯化方法
- Antigen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from Chlamydomonas reinhardtii Cytf (PetA)
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anti-Cyt F antibody
适用: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum, Barley, Ulva prolifera, Synechocystis, Selaginella martensii, Nostoc (Cyanobacteria), Nannochloropsis gaditana, Craterostigma pumilum, Colobanthus quitensis, Chlorella vulgaris, Physcomytrium Patens WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 10 000 - 1: 15 000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution, add 50 μL, of sterile or deionized water.
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缓冲液
- Antigen affinity purified serum, in PBS pH 7.4
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Cyt F
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背景
- Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c'-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
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分子量
- 34 kDa
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UniProt
- P23577
抗原
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