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tdTomato 抗体 (DyLight 680)

The 山羊 多克隆 anti-tdTomato antibody is suitable to detect tdTomato in samples from Discosoma 和 Tomato. It has been validated for IF, IHC (fro) 和 WB.
产品编号 ABIN7830305
发货至: 中国
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Quick Overview for tdTomato 抗体 (DyLight 680) (ABIN7830305)

抗原

tdTomato (tdTomato Fluorescent Protein (tdTomato))

适用

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Discosoma, Tomato

宿主

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山羊

克隆类型

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多克隆

标记

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This tdTomato antibody is conjugated to DyLight 680

应用范围

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Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
  • 原理

    Anti-tdTomato, DyLight®680

    序列

    MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

    特异性

    In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

    产品特性

    Red Fluorescent Protein

    纯化方法

    Epitope affinity purified

    免疫原

    Purified recombinant peptide produced in E. coli

    亚型

    IgG
  • 应用备注

    WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

    限制

    仅限研究用
  • 缓冲液

    PBS, 20 % glycerol and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    The antibody solution should be gently mixed before use.

    储存条件

    4 °C,-20 °C

    储存方法

    For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
  • 抗原

    tdTomato (tdTomato Fluorescent Protein (tdTomato))

    别名

    tdTomato

    背景

    Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight® 680. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

    Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.

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