Ly-49C/I 抗体 (PerCP-Cy5.5)
The 小鼠 单克隆 anti-Ly-49C/I antibody is suitable to detect Ly-49C/I in samples from 小鼠. It has been validated for FACS.
产品编号 ABIN7828904
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Ly-49C/I 抗体 (PerCP-Cy5.5) (ABIN7828904)
抗原
Ly-49C/I
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
PerCP-Cy5.5
应用范围
Flow Cytometry (FACS)
克隆位点
5E6
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原理
- PerCP/Cyanine5.5 Anti-Mouse Ly-49C/I Antibody[5E6]
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亚型
- IgG2a, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Control: (ABIN7469783)
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限制
- 仅限研究用
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浓度
- 5 μL/tests
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缓冲液
- Phosphate buffered solution, pH 7.2, containing 0.09 % stabilizer.
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注意事项
- Please protected from prolonged exposure to light and do not freeze
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储存条件
- 4 °C
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储存方法
- This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
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有效期
- 12 months
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- Ly-49C/I
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背景
- Ly49C,Ly49I,AN006590,The 5E6 (also known as clone SW5E6) antibody reacts with Ly-49C[BALB], Ly-49C[B6], Ly-49C[NZB], and Ly-49I[B6], inhibitory receptors which are expressed on subsets of natural killer (NK) cells and NK-1.1+ (or DX5+) T lymphocytes (NK-T cells) in all strains tested except C57BR and RIII, on a population of memory CD8+ T lymphocytes and NK1.1+ γδ T cells in C57BL/6 mice, and on a distinct subset of B-1 cells of BALB/c and C57BL/6 mice. The proportion of NK T cells expressing Ly-49C/I is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down-regulation of Ly-49 receptor expression is necessary for normal NK T-cell development. Most NK cells express a single allele of Ly-49C, although occasionally they may express more than one allele. The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. The 5E6 antibody is specific for the Ly-49C CRD. The Ly-49C[BALB] and Ly-49C[B6] alloantigens bind to MHC class I antigens of the b, d, k, and s haplotypes, and the 5E6 antibody blocks this binding. Binding of Ly-49C[BALB]- and Ly-49C[B6]- expressing transfectants to lymphoblasts of H-2[f], H-2[q], H-2[r], and H-2[v] strains has also been detected. Ly-49I[B6] transfectants bind H-2[r] lymphoblasts and bind much more weakly to the b, d, k, q, s, and v haplotypes. The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in vivo, and the various levels of expression of an Ly-49 inhibitory receptor may affect the specificity of NK cells. Ly-49C is specifically downregulated in the presence of H-2K[b] class I molecules (one of the Ly-49C ligands). Ly-49C[+] and/or Ly-49I[+] cells mediate allogeneic and hybrid resistance to H-2d bone marrow transplantation. In vitro and in vivo studies suggest that the Ly-49C and/or Ly-49I receptors mediate negative regulation of NK-cell cytolytic activity via tyrosine phosphorylation of their ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs). Cat.No. Product Name Sizes E-CK-A105 10xACK Lysis Buffer 500 mL , 200 mL , 100 mL E-CK-A107 Cell Staining Buffer 500 mL , 200 mL , 100 mL Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [
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基因ID
- 16634, 16640
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UniProt
- Q64329, Q2TJJ8
抗原
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