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Recombinant Cyclin B1 抗体

This 兔 单克隆 antibody specifically detects Cyclin B1 in WB, IF 和 IP. It exhibits reactivity toward 人.
产品编号 ABIN7828138
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for Recombinant Cyclin B1 抗体 (ABIN7828138)

抗原

See all Cyclin B1 (CCNB1) 抗体
Cyclin B1 (CCNB1)

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Cyclin B1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

A546
  • 原理

    Recombinant Cyclin B1 Monoclonal Antibody

    纯化方法

    Protein A purified

    免疫原

    Recombinant human Cyclin B1 fragment

    亚型

    IgG, kappa
  • 应用备注

    WB 1:500-1:1000,IF 1:50,IP 1:50-1:100

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

    有效期

    12 months
  • 抗原

    Cyclin B1 (CCNB1)

    别名

    Cyclin B1

    背景

    G2 mitotic specific cyclin B,G2/mitotic-specific cyclin-B,Cyclin B,CCNB,CCNB1,Cyclin B1,CCNB 1,G2 mitotic specific cyclin B1,G2/mitotic-specific cyclin-B1,Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

    分子量

    Calculated MW: 55 kDa

    Observed MW: 55 kDa

    UniProt

    P14635

    途径

    Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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