Recombinant Cyclin B1 抗体
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北京 101111
Quick Overview for Recombinant Cyclin B1 抗体 (ABIN7828138)
抗原
See all Cyclin B1 (CCNB1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cyclin B1 Monoclonal Antibody
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纯化方法
- Protein A purified
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免疫原
- Recombinant human Cyclin B1 fragment
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亚型
- IgG, kappa
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应用备注
- WB 1:500-1:1000,IF 1:50,IP 1:50-1:100
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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有效期
- 12 months
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- Cyclin B1 (CCNB1)
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别名
- Cyclin B1
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背景
- G2 mitotic specific cyclin B,G2/mitotic-specific cyclin-B,Cyclin B,CCNB,CCNB1,Cyclin B1,CCNB 1,G2 mitotic specific cyclin B1,G2/mitotic-specific cyclin-B1,Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [
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分子量
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Calculated MW: 55 kDa
Observed MW: 55 kDa
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UniProt
- P14635
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途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
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