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Recombinant ZAP70 抗体 (AA 247-382)

This 兔 单克隆 anti-ZAP70 antibody (Clone 870R) specifically detects ZAP70 in IHC. The antibody is reactive with 人 samples.
产品编号 ABIN7826139
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for Recombinant ZAP70 抗体 (AA 247-382) (ABIN7826139)

抗原

See all ZAP70 抗体
ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This ZAP70 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

870R
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    AA 247-382

    原理

    ZAP-70 antibody (870R)

    免疫原

    Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:75-1:150

    说明

    Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.

    Product Description/Background: ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.

    实验流程

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    有效期

    24 months
  • 抗原

    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

    别名

    ZAP-70

    背景

    Gene Alias: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa

    UniProt

    P43403

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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