Peroxiredoxin 4 抗体 (AA 167-260)
Quick Overview for Peroxiredoxin 4 抗体 (AA 167-260) (ABIN7825670)
抗原
See all Peroxiredoxin 4 (PRDX4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 167-260
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原理
- Anti-Zebrafish PRDX4 Antibody
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产品特性
- Anti-Zebrafish PRDX4 Antibody catalog # AZA3KP44. Tested in WB, IHC applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived zebrafish PRDX4 recombinant protein (Position: S167-N260).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Zebrafish
Immunohistochemistry, 2-5 μg/mL, Zebrafish
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Peroxiredoxin 4 (PRDX4)
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别名
- PRDX4
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背景
- PRDX4 (peroxiredoxin 4) is also known as AOE37-2. The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al. suggested PRDX4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.
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分子量
- 27 kDa
抗原
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