Hephaestin 抗体 (AA 348-1157)
Quick Overview for Hephaestin 抗体 (AA 348-1157) (ABIN7825451)
抗原
See all Hephaestin (HEPH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 348-1157
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原理
- Anti-Hephaestin/HEPH Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hephaestin/HEPH Antibody catalog # A05316-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hephaestin/HEPH recombinant protein (Position: E348-Q1157). Mouse Hephaestin/HEPHshares 85.6% and 96.2% amino acid (aa) sequence identity with human and rat Hephaestin/HEPH, respectively.
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亚型
- IgG
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anti-Hephaestin (HEPH) (AA 315-424) antibody
HEPH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D3 unconjugated
anti-Hephaestin (HEPH) (AA 21-120) antibodyHEPH 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Hephaestin (HEPH) (AA 300-580) antibodyHEPH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hephaestin (HEPH) (AA 315-424) antibodyHEPH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Hephaestin (HEPH)
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别名
- HEPH
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背景
- Enables ferroxidase activity. Involved in intestinal iron absorption, multicellular organismal-level iron ion homeostasis, and positive regulation of iron export across plasma membrane. Acts upstream of or within erythrocyte differentiation. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in basolateral plasma membrane. Orthologous to human HEPH (hephaestin).
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分子量
- 130 kDa
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基因ID
- 15203
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UniProt
- Q9Z0Z4
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途径
- Transition Metal Ion Homeostasis
抗原
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