Feimin 抗体
The 兔 多克隆 anti-Feimin antibody is suitable to detect Feimin in samples from 人 和 小鼠. It has been validated for ELISA, IP 和 WB.
产品编号 ABIN7822917
Supplier Product No.: 200-401-nh2
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Feimin 抗体 (ABIN7822917)
抗原
Feimin
适用
人, 小鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
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Supplier Product No.
- 200-401-nh2
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Supplier
- Rockland
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原理
- Anti-Feimin (RABBIT) Antibody
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交叉反应 （详细）
- A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.
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纯化方法
- Anti-Feimin Antibody is purified from monospecific antiserum by Protein A affinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant mouse Feimin protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Feimin (N-Term) antibody
适用: 人 ELISA, IP, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Anti-Feimin (RABBIT) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T and E. coli). A band approximately 20.1kDa in size corresponding to C5orf24 is detected. Cell lines expressing feimin can be expected using appropriate tissues and lysates. Specific conditions for reactivity should be optimized by the end user.
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说明
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ELISA Dilution: 1:44,500-1:64,500
Western Blot Dilution: 40 ng/mL
Immunoprecipitation Dilution: 40 ng/mL -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.18 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Feimin
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背景
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Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin -
基因ID
- 134553
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NCBI登录号
- NP_001129058.1
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UniProt
- Q80X32
抗原
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