Atp1b2/Na+K+Atpase (AA 201-290) 抗体
This anti- antibody (ABIN738426) is a Rabbit Polyclonal antibody detecting in WB, IF (cc), IF (p), ELISA, IHC (fro), ICC. Suitable for Human, Mouse, Rat.
(1 validation)Quick Overview for Atp1b2/Na+K+Atpase (AA 201-290) 抗体 (ABIN738426)
抗原
Atp1b2/Na+K+Atpase
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
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抗原表位
- AA 201-290
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Cow,Pig,Horse,Rabbit,Guinea Pig
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human ATP1b2
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亚型
- IgG
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 -
限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100012
- 日期
- 2016.05.11
- 抗原
- Atp1b2/Na+K+Atpase (ATP1B2)
- Lot Number
- Method validated
- Immunofluorescence
- Positive Control
- Wild-type mouse adult brain cryo-sections
- Negative Control
- Rabbit IgG
- Notes
- The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.
- Primary Antibody
- ABIN738426
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30min (or RT for 30min).
- Wash with PBS 5min X 3 (shake gently).
- Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
- Incubate with ABIN738426 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
- Wash with PBS 5min X 3.
- Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
- Wash with PBS 5min X 3.
- Add three drops of water-based Immu-mount.
- Cover with 24 X 60 cover glass.
- Imaging:
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN738426 worked within 1:50–1:400, worked well at 1:400.
生效 #100012 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of ATP1B2 antibody ABIN738426 on mouse brain sections.
A. ABIN738426 diluted 1:400 (red), DAPI counterstaining (blue).
B. Dilution factor 1:50. C. Dilution factor 1:200. D. Dilution factor 1:400.]()
IF validation of ATP1B2 antibody ABIN738426 on mouse brain sections.
A. ABIN738426 diluted 1:400 (red), DAPI counterstaining (blue).
B. Dilution factor 1:50. C. Dilution factor 1:200. D. Dilution factor 1:400.
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- Atp1b2/Na+K+Atpase
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背景
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Synonyms: AMOG, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, Sodium/potassium-dependent ATPase subunit beta-2, ATP1B2
Background: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.
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基因ID
- 482
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UniProt
- P14415
抗原
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