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CAPN1 抗体 (AA 625-713)

Name: CAPN1 抗体 (AA 625-713)
SKU: ABIN737646
Availability: OutOfStock

Cited in 2+ publications. The 兔多克隆 anti-CAPN1 antibody (ABIN737646) specifically detects CAPN1 in WB, FACS, IHC (p), ELISA, IF (cc), IF (p), IP 和 IHC (fro). The antibody is reactive with 人, 小鼠和大鼠 samples.

Paraformaldehyde-fixed, paraffin embedded mouse liver tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 抗体 (AA 625-713))

IHC (p)

ELISA

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 抗体 (AA 625-713))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 抗体 (AA 625-713))

IHC (p)

4 images

(2 references)

产品编号 ABIN737646

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CAPN1 抗体 (AA 625-713) (ABIN737646)

抗原

See all CAPN1 抗体

CAPN1 (Calpain 1 (CAPN1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CAPN1 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
15

6

5

3

2

1

1

1

1

1

1

1

1

1
AA 625-713

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Sheep,Pig,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Calpain 1

亚型

IgG
anti-Calpain 1 (CAPN1) antibody
CAPN1 适用: 人 WB, ICC, IF, IHC (p), IP 宿主: 兔 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (Middle Region) antibody
CAPN1 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) antibody
CAPN1 适用: 人, 小鼠, 大鼠, Cow WB, IHC, IHC (p), ELISA, IP 宿主: 小鼠 Monoclonal 15C10 unconjugated

anti-Calpain 1 (CAPN1) antibody
KD Validated CAPN1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3290 unconjugated Recombinant Antibody

anti-Calpain 1 (CAPN1) (AA 468-481) antibody
CAPN1 适用: 人, Cow, Pig, 山羊, 兔, 豚鼠, 猴, 非洲爪蟾 WB, IHC, IHC (p), ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 515-526) antibody
CAPN1 适用: 人 WB, IHC, IHC (p), ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 300-349) antibody
CAPN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 山羊, 兔, 绵羊 WB 宿主: 兔 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 185-234) antibody
CAPN1 适用: 人, Cow, Pig, 山羊, 兔, 绵羊, 豚鼠, 猴, 非洲爪蟾, Bat, 小鸡, 犬, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 625-713) antibody (Biotin)
CAPN1 适用: 人, 小鼠, 大鼠 WB, IHC (p), ELISA, IP, IHC (fro) 宿主: 兔 Polyclonal Biotin

anti-Calpain 1 (CAPN1) (AA 625-713) antibody (AbBy Fluor® 750)
CAPN1 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 750

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Li, Chen, Lu, Zhang, Guan, Li, Wang: "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model." in: International journal of molecular sciences, Vol. 18, Issue 8, (2017) (PubMed).

Miners, Renfrew, Swirski, Love: "Accumulation of ¿-synuclein in dementia with Lewy bodies is associated with decline in the ¿-synuclein-degrading enzymes kallikrein-6 and calpain-1." in: Acta neuropathologica communications, Vol. 2, Issue 1, pp. 164, (2014) (PubMed).
抗原

CAPN1 (Calpain 1 (CAPN1))

别名

Calpain 1

背景

Synonyms: Capa1, Capa-1, mu-calpin, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain, muCANP, Capn1, Canp1
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

基因ID

12333

UniProt

O35350