Recombinant CD32/CD16 抗体
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北京 101111
Quick Overview for Recombinant CD32/CD16 抗体 (ABIN7795365)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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Expression System
- Mammalian cells
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原理
- In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Mouse IgG2a LALAPG Kappa (Clone: 2.4G2)
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产品特性
- The recombinant 2.4G2 antibody binds to mouse CD16 (FcγIII) and CD32 (FcγII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (FcγI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications. The most popular mouse Fc blocking reagents include: Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining, it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity. Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls. Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking. Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. Best choice of mouse Fc blocking reagents based on your application are: For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2). For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. For Western Blot and ELISA: BSA or FBS. For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.
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纯化方法
- Protein A or G affinity column
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纯度
- >95 % by reducing SDS-PAGE
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内毒素水平
- < 1 EU per 1 mg of the protein by the LAL method
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亚型
- IgG2a, L234A, L235A, P329G
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anti-CD32/CD16 (CD32/CD16) antibody
CD32/CD16 适用: 小鼠 FACS, IP, IHC, IF, BR 宿主: 大鼠 Monoclonal 2-4G2 unconjugated Recombinant Antibody
anti-CD32/CD16 (CD32/CD16) antibodyCD32/CD16 适用: 小鼠 FACS, IP 宿主: 大鼠 Monoclonal 93 unconjugated
anti-CD32/CD16 (CD32/CD16) antibody (PE)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 PE
anti-CD32/CD16 (CD32/CD16) antibody (FITC)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 FITC
anti-CD32/CD16 (CD32/CD16) antibody (Cy7,PE)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 Cy7,PE
anti-CD32/CD16 (CD32/CD16) antibody (PE-Cy7)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 PE-Cy7
anti-CD32/CD16 (CD32/CD16) antibody (SPRD)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 SPRD
anti-CD32/CD16 (CD32/CD16) antibody (PE-Cy5.5)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 PE-Cy5.5
anti-CD32/CD16 (CD32/CD16) antibody (Biotin)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 Biotin
anti-CD32/CD16 (CD32/CD16) antibody (APC)CD32/CD16 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 93 APC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- > 3 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.
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- CD32/CD16
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别名
- CD16/CD32
抗原
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