Recombinant CD33 (Gemtuzumab Biosimilar) 抗体
Quick Overview for Recombinant CD33 (Gemtuzumab Biosimilar) 抗体 (ABIN7795111)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
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Expression System
- Mammalian cells
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原理
- Gemtuzumab Biosimilar, CD33 Monoclonal Antibody
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产品特性
- Gemtuzumab is a recombinant humanized IgG4 kappa antibody which is conjugated with calicheamicin derivative, a cytotoxic antitumor antibiotic isolated from fermentation of Micromonospora echinospora ssp. calichensis. Gemtuzumab ozogamicin has approximately 50 % of the antibody loaded with 4-6 Moles calicheamicin per mole of antibody Label. The antibody is specifically directed against the CD33 antigen present on leukemic myeloblasts in most patients with acute myeloid leukemia (AML). By binding to the CD33 antigen on tumors, the cytotoxic agent blocks the growth of cancerous cells and causes cell death. Gemtuzumab is directed against the CD33 antigen expressed by hematopoietic cells. Binding of the anti-CD33 antibody portion of Gemtuzumab with the CD33 antigen results in the formation of a complex that is internalized. Upon internalization, the calicheamicin derivative is released inside the lysosomes of the myeloid cell. The released calicheamicin derivative binds to DNA in the minor groove resulting in site-specific DNA double strand breaks via formation of a p-benzene diradical. Eventually, cell death is induced.
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纯化方法
- Protein A or G affinity column
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纯度
- >95 % by reducing SDS-PAGE
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内毒素水平
- < 1 EU per 1 mg of the protein by the LAL method
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免疫原
- Human CD33
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anti-CD33 (Gemtuzumab Biosimilar) antibody
Research Grade 适用: 人 FACS 宿主: Humanized Monoclonal unconjugated Recombinant Antibody
anti-CD33 (Gemtuzumab Biosimilar) antibody适用: 人 FACS, IF 宿主: 兔 Chimeric HP67-6 unconjugated Recombinant Antibody
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- > 3 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.
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- CD33 (Gemtuzumab Biosimilar)
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别名
- Gemtuzumab Biosimilar
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物质类
- Biosimilar
抗原
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