SARS-CoV-2 Spike 抗体 (RBD)
Quick Overview for SARS-CoV-2 Spike 抗体 (RBD) (ABIN7794950)
抗原
See all SARS-CoV-2 Spike 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- RBD
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原理
- Anti-SARS-CoV-2 Spike RBD (Clone: 2381) Purified No Carrier Protein
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特异性
- Anti-SARS-CoV-2 Spike RBD, clone 2381, specifically targets an epitope on the SARS-CoV-2 spike protein receptor-binding domain (RBD). Antigen Distribution The spike RBD is expressed on the surface of SARS-CoV-2. Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6 % identity with SARS-CoV, encodes four essential structural proteins the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). The CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells 3-5. Although both SARS-CoV and SARS-CoV-2 bind the ACE2 receptor, the RBDs only share ~73 % amino acid identity, and the SARS-CoV-2 RBD binds with a higher affinity compared to SARS-CoV 3, 6. The RBD is dynamic and undergoes hinge-like conformational changes, referred to as the ?down? or ?up? conformations, which hide or expose the receptor-binding motifs, respectively 7. Following receptor binding, S1 destabilizes, and TMPRSS2 cleaves S2, which undergoes a pre- to post-fusion conformation transition, allowing for membrane fusion 8, 9. Polyclonal RBD-specific antibodies can block ACE2 binding 10, 11, and anti-RBD neutralizing antibodies are present in the sera of convalescent COVID19 patients 12, identifying the RBD as an attractive candidate for vaccines and therapeutics. In addition, the RBD is poorly conserved, making it a promising antigen for diagnostic tests 13 14. Serologic tests for the RBD are highly sensitive and specific for detecting SARS-CoV-2 antibodies in COVID19 patients 13 15. Furthermore, the levels of anti-RBD antibodies correlated with SARS-CoV-2 neutralizing antibodies, suggesting the RBD could be used to predict an individual's risk of disease 13.
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纯化方法
- Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
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纯度
- >=90 % monomer by analytical SEC and SDS-Page
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亚型
- IgG1
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anti-SARS-CoV-2 Spike (C-Term) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Gamma ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (AA 319-541) antibodyAnimal-Free 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IF 宿主: 小鼠 Monoclonal AB68-A09 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 319-541), (Receptor Binding Domain) antibody (Fc Tag)适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut, GICA 宿主: Human Monoclonal A1 Fc Tag single-domain Antibody (sdAb) Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: Human Monoclonal AM004414 (414-4) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal AM015553 (553-15) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 319-541) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM54 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 16-685) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, GICA 宿主: Chimeric Chimeric H6 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 16-685) antibody (Fc Tag,Biotin)适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: 小鼠 Monoclonal H6 Fc Tag,Biotin scFv fragment Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 16-685) antibody (Fc Tag,HRP)适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: 小鼠 Monoclonal H6 Fc Tag,HRP scFv fragment Recombinant Antibody
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限制
- 仅限研究用
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浓度
- 1 μg/mL
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缓冲液
- This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
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储存液
- Without preservative
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储存条件
- 4 °C,-80 °C
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储存方法
- This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at <= -70°C.Avoid Repeated Freeze Thaw Cycles.
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- SARS-CoV-2 Spike
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物质类
- Viral Protein
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背景
- COV2-2381, SARS-CoV-2 Spike RBD Antibody, Receptor Binding Domain Antibody,
抗原
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