SARS-CoV-2 Nucleocapsid 抗体
Quick Overview for SARS-CoV-2 Nucleocapsid 抗体 (ABIN7794936)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-B9) Purified No Carrier Protein
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特异性
- Anti-SARS-CoV-2 Nucleocapsid, clone NP1-B9, specifically targets an epitope on the SARS-CoV-2 nucleocapsid protein. Futhermore, it is reported to bind to the oligomerization domain of the N protein. Antigen Distribution The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2. Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus belonging to the Coronaviridae family1. The SARS-CoV-2 genome encodes four essential proteins the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins2. SARS-CoV-2 shares 79.6 % identity with the original SARS-CoV2. The N protein is 46 kDa and consists of two highly conserved structural domains, the N-terminal domain (NTD) and C-terminal domain (CTD), connected by a linker region. The NTD and CTD are involved in a couple of primary functions, including RNA binding and self-oligomerization3,4. This results in binding to and packaging of the viral RNA genome into a helical ribonucleoprotein5. The N protein is involved in other critical steps of the viral life cycle, including transcription, replication, and modulating infected cell signaling pathways6,7. The N protein is a suitable candidate for vaccine development and diagnostic assays8 for several reasons. It is abundantly expressed during infection and is highly conserved, sharing 90 % amino acid homology with the SARS-CoV N protein9. Furthermore, antibodies9,10 and memory T cells11,12 targeting the N protein are identified in the sera of convalescent COVID-19 patients, demonstrating it as immunogenic. The N protein also suppresses antiviral RNAi, evading the innate immune system13, suggesting its potential value as a targeted therapeutic.
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纯化方法
- Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
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纯度
- >=90 % monomer by analytical SEC and SDS-Page
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亚型
- IgG1
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anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (Whole Molecule) antibody
Animal-Free SARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, IF, FACS 宿主: 小鼠 Monoclonal AB79-E11 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, IF 宿主: 兔 Chimeric CR3018 (03-018) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, GICA 宿主: 小鼠 Monoclonal 3C6 unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2), Virus ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (C-Term) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
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限制
- 仅限研究用
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浓度
- 1 μg/mL
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缓冲液
- This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
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储存液
- Without preservative
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储存条件
- 4 °C,-80 °C
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储存方法
- This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at <= -70°C.Avoid Repeated Freeze Thaw Cycles.
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-2 Nucleocapsid (N)
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物质类
- Viral Protein
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背景
- COV2-NP1-B9, SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein, Protein N, SARS-CoV N Protein,
抗原
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