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CD86 抗体 (AA 140-175)

Name: CD86 抗体 (AA 140-175)
SKU: ABIN736701
Availability: OutOfStock

This anti-CD86 antibody is a 兔多克隆 antibody detecting CD86 in FACS, WB, ELISA, IHC (fro), ICC, IHC (p), IF (p) 和 IF (cc). Suitable for 人, 小鼠, 大鼠和犬. This Primary Antibody has been cited in 15+ publications.

HeLa cell lysates probed with Rabbit Anti-CD86 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C. (CD86 抗体 (AA 140-175))

Formalin-fixed and paraffin embedded rat lung labeled with Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated (ABIN736701) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD86 抗体 (AA 140-175))

IHC

L1 rat brain lysates L2 rat heart lysates probed with Anti CD86/B7-2 Polyclonal Antibody, Unconjugated (ABIN736701) at 1:200 overnight at 4 °C. (CD86 抗体 (AA 140-175))

SDS

Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with Anti-CD86/B7-2 Polyclonal Antibody , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD86 抗体 (AA 140-175))

IHC (p)

Chronic phase glibenclamide reduces inflammation and improves the macrophage phenotype in EAE. (CD86 抗体 (AA 140-175))

IHC (p)

5 images

(15 references)

产品编号 ABIN736701

发货至: 中国

Quick Overview for CD86 抗体 (AA 140-175) (ABIN736701)

抗原

See all CD86 抗体

CD86

适用

人, 小鼠, 大鼠, 犬

宿主

兔

克隆类型

多克隆

标记

This CD86 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

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AA 140-175

交叉反应

犬, 人, 小鼠, 大鼠

预测反应

Cow,Sheep,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from rat CD86

亚型

IgG
anti-CD86 (CD86) (AA 37-272) antibody
CD86 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD86 (CD86) (AA 30-247) antibody
CD86 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, IF, ELISA, BP, StM, Coat 宿主: 兔 Monoclonal C86-2160R unconjugated Recombinant Antibody

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, Func, BP, StM 宿主: 小鼠 Monoclonal BU63 unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal BU63 unconjugated

anti-CD86 (CD86) antibody (FITC)
CD86 适用: 人 FACS 宿主: 小鼠 Monoclonal BU63 FITC

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 WB, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal C86-1146 unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal rC86-1146 unconjugated Recombinant Antibody

anti-CD86 (CD86) antibody
CD86 适用: 人 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal BU63 unconjugated

anti-CD86 (CD86) antibody (PerCP)
CD86 适用: 人 FACS 宿主: 小鼠 Monoclonal BU63 PerCP

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Diehl, Kirchhoff, Hansmann: "CD86 is linked to apoptosis in canine histiocytic sarcoma." in: Frontiers in veterinary science, Vol. 12, pp. 1546047, (2025) (PubMed).

Diehl, Hansmann: "Immune checkpoint regulation is critically involved in canine cutaneous histiocytoma regression." in: Frontiers in veterinary science, Vol. 11, pp. 1371931, (2024) (PubMed).

Choi, Mangal, Park, Kim, Jun, Jung, Choi, Jung, Lee, Na, Ryu, Kim, Kwon, Koh, Lee, Hwang, Lee, Jung, Cha, Choi, Hong: "Occlusive membranes for guided regeneration of inflamed tissue defects." in: Nature communications, Vol. 14, Issue 1, pp. 7687, (2023) (PubMed).

Lee, Yu, Trimpert, Benthani, Mairhofer, Richter-Pechanska, Wyler, Belenki, Kaltenbrunner, Pammer, Kausche, Firsching, Dietert, Schotsaert, Martínez-Romero, Singh, Kunz, Niemeyer, Ghanem, Salzer, Paar et al.: "Virus-induced senescence is a driver and therapeutic target in COVID-19. ..." in: Nature, Vol. 599, Issue 7884, pp. 283-289, (2021) (PubMed).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, (2018) (PubMed).

Chen, Zhang, Wang, Chen, Yan, Zhang, Zhang: "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." in: American journal of translational research, Vol. 9, Issue 5, pp. 2352-2362, (2017) (PubMed).

Zhang, Zhang, Yin, Li, Bao, Chen: "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." in: Oncology letters, Vol. 13, Issue 4, pp. 2297-2303, (2017) (PubMed).

Gerzanich, Makar, Guda, Kwon, Stokum, Woo, Ivanova, Ivanov, Mehta, Morris, Bryan, Bever, Simard: "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 177, (2017) (PubMed).

Xie, Wu, Zhou, Yang, Tian, Huang, Meng, Li: "PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization." in: Biochimie, Vol. 127, pp. 121-32, (2016) (PubMed).

Das, Singh, Majid, Sherman, Mukhopadhyay, Wright, Martin, Dunn, Baker: "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." in: Advanced healthcare materials, Vol. 5, Issue 17, pp. 2248-60, (2016) (PubMed).

Chen, Li, Zhao, Liang, Ma, Li, Zhu: "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 202-8, (2016) (PubMed).

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, (2016) (PubMed).

Chen, Shi, Zhang, Chen, Wang, Zhang, Tian, Yan, Li, Zhong, Xing, Zhang, Zhang: "Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing." in: Scientific reports, Vol. 5, pp. 18104, (2016) (PubMed).

Evans, Jonason, Bussler, Torno, Veeraraghavan, Reilly, Doherty, Seils, Winter, Mallow, Kirk, Howell, Giralico, Scrivens, Klimatcheva, Fisher, Bowers, Paris, Smith, Zauderer: "Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies." in: Cancer immunology research, (2015) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).
抗原

CD86

别名

CD86

背景

Synonyms: B7-2, Cd86 antigen, Cd86, Membrane glycoprotein
Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

基因ID

56822

UniProt

O35531

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation