Recombinant Vimentin 抗体
Quick Overview for Recombinant Vimentin 抗体 (ABIN7674794)
抗原
See all Vimentin (VIM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant mouse anti vimentin
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特异性
- Human and mouse vimentin
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产品特性
- Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
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纯化方法
- ProtA purified
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免疫原
- This clone RV204 is a recombinant mouse anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV204 is produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
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亚型
- IgG1
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) (AA 1-200) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody
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应用备注
- This clone RV204 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
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限制
- 仅限研究用
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溶解方式
- Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0.09 % sodium azide. The purity of the antibody is >98 % as determined by SDS-Polyacrylamide gel electrophoresis. The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.
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储存条件
- 4 °C,-20 °C
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储存方法
- The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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- Vimentin (VIM)
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别名
- vimentin
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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