MBP Tag 抗体
Quick Overview for MBP Tag 抗体 (ABIN7673460)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MBP tag antibody
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纯化方法
- Protein A+G purification
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
- MBP-Tag
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亚型
- IgG2a
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anti-MBP Tag antibody
WB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibody适用: 标记 WB, ELISA, IP, ICC, IHC, FACS 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
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应用备注
- WB: 1:1000-1:8000, IP: 1:5000-1:50000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
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有效期
- 12 months
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- MBP Tag
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物质类
- Tag
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背景
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Synonyms: Maltose Binding Protein|MBP|MBP Tag
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
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分子量
- 40 kDa
抗原
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