GFP Tag 抗体 (AA 1-238)
Quick Overview for GFP Tag 抗体 (AA 1-238) (ABIN7673314)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-238
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原理
- GFP tag antibody
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纯化方法
- Protein A+G purification
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
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Immunogen sequence: 1-238aa
Immunogen: GFP tag
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亚型
- IgG1
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anti-GFP Tag antibody
适用: 人 WB, IF 宿主: 小鼠 Monoclonal 168AT1211 unconjugated
anti-GFP Tag antibodyWB 宿主: 小鼠 Monoclonal 168AT1211-269-64 unconjugated
anti-GFP Tag antibodyWB 宿主: 兔 Polyclonal unconjugated
anti-GFP Tag (N-Term) antibody适用: Aequorea victoria WB, ELISA, IHC 宿主: 小鼠 Monoclonal LGB-1 unconjugated
anti-GFP Tag antibody适用: Aequorea victoria ELISA, IF, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB 宿主: 小鼠 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:2000-1:16000, IP: 1:1000-1:4000, IF: 1:20-1:200
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
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有效期
- 12 months
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- GFP Tag
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物质类
- Tag
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背景
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Synonyms: CFP|eGFP|eYFP|GFP|GFP tag|YFP
Background: Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9 kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
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分子量
- 30 kDa
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UniProt
- P42212
抗原
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