Testosterone 抗体 (CF®514)
This anti-Testosterone antibody is a 小鼠 单克隆 antibody detecting Testosterone in IHC, RIA 和 IHC (fp). Suitable for Various Species.
Quick Overview for Testosterone 抗体 (CF®514) (ABIN7660683)
抗原
See all Testosterone 抗体
Testosterone
适用
Various Species
宿主
小鼠
克隆类型
单克隆
标记
This Testosterone antibody is conjugated to CF®514
应用范围
Immunohistochemistry (IHC), Radioimmunoassay (RIA), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
克隆位点
4E1G2
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原理
- Testosterone(4E1G2), CF514 conjugate
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产品特性
- This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone <0.1%, 5-alpha DHT 0.8%, estradiol <0.1% and progesterone <0.1%. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Testosterone 3 CMO conjugated to BSA
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亚型
- IgG1, kappa
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anti-Testosterone antibody
ELISA, IHC, Coat, RIA, StM 宿主: 小鼠 Monoclonal 4E1G2 unconjugated
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应用备注
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology formalin-paraffin 0.25-0.5 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Interstitial or Leydid cells in testis.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Testosterone
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物质类
- Hormone
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背景
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Synonyms: 17i²-Hydroxy-3-oxo-4-androstene, 17i²-Hydroxy-4-androsten-3-one, 4-Androsten-17i²-ol-3-one, trans-Testosterone
Tissue Expression: Testis
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分子量
- 288.42Da
抗原
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