Spastin 抗体 (CF®594)
Quick Overview for Spastin 抗体 (CF®594) (ABIN7660084)
抗原
See all Spastin (SPAST) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Spastin(Sp 6C6), CF594 conjugate
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产品特性
- The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human Spastin protein.
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亚型
- IgG2a, kappa
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anti-Spastin (SPAST) (AA 200-304) antibody
SPAST 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Spastin (SPAST) (AA 551-616) antibodySPAST 适用: 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) antibodySPAST 适用: 小鼠, 大鼠, 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) (AA 200-304) antibodySPAST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Spastin (SPAST) antibodySPAST 适用: 小鼠, 大鼠, 人 WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal Sp 3G11-1 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.
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说明
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Positive Control: HeLa cells. K-562 or Raji cell lysates.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Spastin (SPAST)
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别名
- Spastin
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背景
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Synonyms: ADPSP, FSP2, Spast, Spatin, SPG4
Gene Symbol: SPAST
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分子量
- 52 kDa
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基因ID
- 6683
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途径
- Microtubule Dynamics, M Phase, Regulation of Cell Size
抗原
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