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Recombinant CD43 抗体 (CF®647)

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, WB, IHC, IF 和 IHC (fp). Suitable for 人.
产品编号 ABIN7659900
发货至: 中国

Quick Overview for Recombinant CD43 抗体 (CF®647) (ABIN7659900)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is conjugated to CF®647

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

RSPN-1094
  • 原理

    CD43 (T-Cell Marker) (rSPN/1094), CF647 conjugate

    产品特性

    This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human CD43 protein

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Positive Control: K562 cells, Paracortex in a tonsil or a reactive lymph node.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    CD43

    背景

    Synonyms: Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

    Gene Symbol: SPN

    Tissue Expression: Lymphocytes

    分子量

    95, 115, or 135 kDa

    基因ID

    6693

    UniProt

    P16150

    途径

    Regulation of Leukocyte Mediated Immunity
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