SELPLG 抗体 (CF®740)
Quick Overview for SELPLG 抗体 (CF®740) (ABIN7659763)
抗原
See all SELPLG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CD162 (Selectin P Ligand) (PSGL1/1601), CF740 conjugate
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产品特性
- CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human full-length SELPLG (CD162) protein
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亚型
- IgG1
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anti-Selectin P Ligand (SELPLG) (AA 251-350) antibody
SELPLG 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibody (PE)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 PE
anti-Selectin P Ligand (SELPLG) (AA 40-280) antibodySELPLG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) (AA 94-345) antibodySELPLG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibodySELPLG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal PSGL1-1601 unconjugated
anti-Selectin P Ligand (SELPLG) (N-Term) antibodySELPLG 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibody (FITC)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 FITC
anti-Selectin P Ligand (SELPLG) antibody (APC)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 APC
anti-Selectin P Ligand (SELPLG) (N-Term) antibodySELPLG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibodySELPLG 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 3G11 unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Jurkat cells. Tonsil or Spleen
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- SELPLG (Selectin P Ligand (SELPLG))
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别名
- CD162
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背景
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Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein lig, 1, PSGL1, Selectin P lig, SELPLG
Gene Symbol: SELPLG
Tissue Expression: Myeloid cells|T-cells
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分子量
- 43-120 kDa
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基因ID
- 6404
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UniProt
- Q14242
抗原
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