Recombinant s100a4 抗体 (AA 1-200) (CF®740)
Quick Overview for Recombinant s100a4 抗体 (AA 1-200) (CF®740) (ABIN7659709)
抗原
See all s100a4 (S100A4) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            抗原表位
- AA 1-200
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                                            原理
- S100A4 (Marker of Tumor Metastasis) (S100A4/2750R), CF740 conjugate
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                                            产品特性
- S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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                                            免疫原
- Recombinant fragment of human S100A4 protein (around aa1-200)
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                                            亚型
- IgG
 
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                                            应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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                                            说明
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                        Positive Control: HeLa, A549 or A375 Cells. Placenta 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- 0.1 mg/mL
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                                            缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            注意事项
- Protect from light
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                                            储存条件
- 4 °C
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                                            储存方法
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                        Stable at room temperature or 37°C for 7 days. Protect from light Store at 2 to 8°C. Protect fluorescent conjugates from light 
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                                            有效期
- 24 months
 
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    - s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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                                            别名
- S100A4
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                                            背景
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                        Synonyms: S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein Gene Symbol: S100A4 
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                                            分子量
- 10-12 kDa
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                                            基因ID
- 6275
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                                            UniProt
- P26447
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                                            途径
- S100 Proteins
 抗原
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