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Recombinant s100a4 抗体 (AA 1-200) (CF®640R)

This anti-s100a4 antibody is a 兔 单克隆 antibody detecting s100a4 in WB, IHC, IF, FACS 和 IHC (fp). Suitable for 人 和 小鼠.
产品编号 ABIN7659698
发货至: 中国

Quick Overview for Recombinant s100a4 抗体 (AA 1-200) (CF®640R) (ABIN7659698)

抗原

See all s100a4 (S100A4) 抗体
s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

抗体类型

Recombinant Antibody

适用

  • 133
  • 88
  • 36
  • 16
  • 7
人, 小鼠

宿主

  • 101
  • 51
  • 1
  • 1
  • 1

克隆类型

  • 80
  • 75
单克隆

标记

  • 62
  • 19
  • 10
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This s100a4 antibody is conjugated to CF®640R

应用范围

  • 118
  • 88
  • 67
  • 60
  • 31
  • 28
  • 23
  • 16
  • 15
  • 13
  • 13
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

S100A4-2750R
  • 抗原表位

    • 29
    • 16
    • 12
    • 11
    • 11
    • 4
    • 2
    • 1
    • 1
    AA 1-200

    原理

    S100A4 (Marker of Tumor Metastasis) (S100A4/2750R), CF640R conjugate

    产品特性

    S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant fragment of human S100A4 protein (around aa1-200)

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: HeLa, A549 or A375 Cells. Placenta

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

    别名

    S100A4

    背景

    Synonyms: S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein

    Gene Symbol: S100A4

    分子量

    10-12 kDa

    基因ID

    6275

    UniProt

    P26447

    途径

    S100 Proteins
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