Recombinant PSAP 抗体 (CF®647)
This anti-PSAP antibody is a 小鼠 单克隆 antibody detecting PSAP in IHC 和 IHC (fp). Suitable for 人.
Quick Overview for Recombinant PSAP 抗体 (CF®647) (ABIN7659219)
抗原
PSAP
(Prostate Specific Acid Phosphatase (PSAP))
抗体类型
Recombinant Antibody
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This PSAP antibody is conjugated to CF®647
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
克隆位点
RACPP-1338
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原理
- Prostate Specific Acid Phosphatase (PSAP) (rACPP/1338), CF647 conjugate
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产品特性
- This antibody recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human ACPP protein
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亚型
- IgG1, kappa
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: PC12 cells or normal prostate or prostate carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- PSAP (Prostate Specific Acid Phosphatase (PSAP))
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别名
- Prostate-Specific Acid Phosphatase
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背景
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Synonyms: 5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase)
Gene Symbol: ACPP
Tissue Expression: Prostate
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分子量
- 52 kDa
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基因ID
- 55
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UniProt
- P15309
抗原
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