Plakophilin 1 抗体 (N-Term) (CF®740)
Quick Overview for Plakophilin 1 抗体 (N-Term) (CF®740) (ABIN7658623)
抗原
See all Plakophilin 1 (PKP1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Plakophilin-1 (PKP1) (10B2), CF740 conjugate
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产品特性
- This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- N-terminus of human Plakophilin-1 protein
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亚型
- IgG1
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anti-Plakophilin 1 (PKP1) (AA 235-726) antibody
PKP1 适用: 人, 小鼠 WB, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 19F10 unconjugated
anti-Plakophilin 1 (PKP1) (N-Term) antibodyPKP1 适用: 人, 小鼠 WB, IF, FACS 宿主: 小鼠 Monoclonal 10B2 unconjugated
anti-Plakophilin 1 (PKP1) (AA 500-742) antibodyPKP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 1 (PKP1) (AA 1-726) antibodyPKP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HaCat Cells.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Plakophilin 1 (PKP1)
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别名
- Plakophilin-1
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背景
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Synonyms: B6P, B, 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)
Gene Symbol: PKP1
Tissue Expression: Cardiac
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分子量
- 75 kDa
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基因ID
- 5317
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UniProt
- Q13835
抗原
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