Recombinant ADRP 抗体 (AA 249-376) (CF®640R)
Quick Overview for Recombinant ADRP 抗体 (AA 249-376) (CF®640R) (ABIN7658553)
抗原
See all ADRP (PLIN2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 249-376
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原理
- Adipophilin / Perilipin-2 (Marker of Lipid Accumulation) (ADFP/2755R), CF640R conjugate
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产品特性
- Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein
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亚型
- IgG
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- ADRP (PLIN2) (Perilipin 2 (PLIN2))
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别名
- Adipophilin
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背景
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Synonyms: Perilipin-2, Adipophilin, ADFP, ADRP, Adipose differentiation-related protein, Perilipin-2
Gene Symbol: PLIN2
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分子量
- 48 kDa
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基因ID
- 123
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UniProt
- Q99541
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途径
- Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
抗原
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