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PAX7 抗体 (AA 300-600) (CF®740)

The CF®740-conjugated 小鼠 单克隆 anti-PAX7 antibody (Clone PAX7-1187) (ABIN7658346) specifically detects PAX7 in IHC 和 IHC (fp). The antibody is reactive with 人 samples.
产品编号 ABIN7658346
发货至: 中国
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Quick Overview for PAX7 抗体 (AA 300-600) (CF®740) (ABIN7658346)

抗原

See all PAX7 抗体
PAX7 (Paired Box 7 (PAX7))

适用

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  • 1
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宿主

  • 83
  • 40
  • 1
  • 1
小鼠

克隆类型

  • 66
  • 59
单克隆

标记

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  • 8
  • 4
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This PAX7 antibody is conjugated to CF®740

应用范围

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  • 21
  • 15
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  • 9
  • 2
  • 1
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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

PAX7-1187
  • 抗原表位

    • 22
    • 16
    • 9
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    • 7
    • 7
    • 6
    • 3
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    • 2
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    • 2
    • 1
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    • 1
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    • 1
    • 1
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    • 1
    • 1
    AA 300-600

    原理

    PAX7 (PAX7/1187), CF740 conjugate

    产品特性

    The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant fragment (aa300-600) of human PAX7 protein

    亚型

    IgG1, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Rhabdomyosarcoma (RMS) or Testicular Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    PAX7 (Paired Box 7 (PAX7))

    别名

    PAX7

    背景

    Synonyms: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

    Gene Symbol: PAX7

    分子量

    57 kDa

    基因ID

    5081

    UniProt

    P23759
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