PAX7 抗体 (AA 300-600) (CF®740)
Quick Overview for PAX7 抗体 (AA 300-600) (CF®740) (ABIN7658346)
抗原
See all PAX7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 300-600
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原理
- PAX7 (PAX7/1187), CF740 conjugate
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产品特性
- The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment (aa300-600) of human PAX7 protein
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亚型
- IgG1, kappa
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Rhabdomyosarcoma (RMS) or Testicular Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- PAX7 (Paired Box 7 (PAX7))
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别名
- PAX7
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背景
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Synonyms: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2
Gene Symbol: PAX7
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分子量
- 57 kDa
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基因ID
- 5081
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UniProt
- P23759
抗原
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