NKX6-1 抗体 (CF®594)
Quick Overview for NKX6-1 抗体 (CF®594) (ABIN7658054)
抗原
See all NKX6-1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NKX6.1 (Marker for Pancreatic and Duodenal Neuroendocrine Tumors) (NKX61/2561), CF594 conjugate
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产品特性
- Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature B cells, which produce Insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic B cell specific genes, including Insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in B cells.Nkx6.1 is highly expressed in pancreatic and duodenal well-differentiated neuroendocrine tumors (WDNETS) and in metastatic WDNETS, is a highly specific marker of tumors of pancreatic origin. It has thus been suggested that Nkx6.1 is a useful inclusion into IHC panels for identifying primary sites of WDNETS.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human full-length recombinant NKX6.1 protein
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亚型
- IgG2c, kappa
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anti-NK6 Homeobox 1 (NKX6-1) antibody
NKX6-1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal NKX61-2561 unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (AA 1-367) antibodyNKX6-1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (Internal Region) antibodyNKX6-1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (AA 191-275) antibodyNKX6-1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-NK6 Homeobox 1 (NKX6-1) antibodyNKX6-1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (AA 285-314) antibodyNKX6-1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (N-Term) antibodyNKX6-1 适用: 人, 小鼠, 大鼠, 犬, 马, Pig, 兔, Cow, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (N-Term) antibodyNKX6-1 适用: 人, 小鼠, 大鼠, 犬, 马, Pig, 兔, Cow, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (AA 284-312) antibodyNKX6-1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NK6 Homeobox 1 (NKX6-1) (AA 284-314), (C-Term) antibodyNKX6-1 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Human fetal small intestine lysate or pancreas tissue.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- NKX6-1 (NK6 Homeobox 1 (NKX6-1))
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别名
- NKX6.1
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背景
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Synonyms: Homeobox protein NK-6 homolog A, Homeobox protein Nkx-6.1, NK homeo box, family 6, member A, NK homeobox (Drosophila), family 6, A, NK homeobox, family 6, A, NK6 homeobox 1, NK6 transcription factor homolog A, NK6 transcription factor related, locus 1, NK6 transcription factor related, locus 1 (Drosophila), NKX6-1, NKX6.1, NKX61, NKX61_HUMAN, NKX6A
Gene Symbol: NKX6-1
Tissue Expression: Pancreatic beta cells
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分子量
- 40-50 kDa
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基因ID
- 4825
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UniProt
- P78426
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途径
- Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion
抗原
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