Myogenin 抗体 (CF®405S)
Quick Overview for Myogenin 抗体 (CF®405S) (ABIN7657635)
抗原
See all Myogenin (MYOG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Myogenin (Skeletal Muscle Marker) (MYOG/2660), CF405S conjugate
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产品特性
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human myogenin (MYOG) protein
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亚型
- IgG1, kappa
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anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 4-219) antibody
MYOG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2B7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E19 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-30) antibodyMYOG 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (Internal Region) antibodyMYOG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (C-Term) antibodyMYOG 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 30-224) antibodyMYOG 适用: 人 WB, ELISA, IF, IP, IHC (p), GS 宿主: 小鼠 Monoclonal F12B unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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别名
- Myogenin
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背景
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Synonyms: bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4
Gene Symbol: MYOG
Tissue Expression: Muscle
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分子量
- 34 kDa
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基因ID
- 4656
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UniProt
- P15173
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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