Recombinant Moesin 抗体 (CF®405S)
Quick Overview for Recombinant Moesin 抗体 (CF®405S) (ABIN7657217)
抗原
See all Moesin (MSN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Moesin (rMSN/492), CF405S conjugate
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产品特性
- This antibody recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human Moesin protein
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亚型
- IgG1, kappa
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Western Blot 0.5-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HT-29, Jurkat, CH3LC, or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Moesin (MSN)
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别名
- Moesin
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背景
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Synonyms: Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSLK fusion
Gene Symbol: MSN
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分子量
- 78 kDa
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基因ID
- 4478
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UniProt
- P26038
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途径
- Asymmetric Protein Localization
抗原
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