MHC, Class I 抗体 (CF®740)
Quick Overview for MHC, Class I 抗体 (CF®740) (ABIN7657101)
抗原
See all MHC, Class I 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MHC I(246-B8.E7), CF740 conjugate
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产品特性
- Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human peripheral blood leukocytes
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亚型
- IgG2a, kappa
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anti-MHC, Class I antibody
适用: 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 绵羊, Cat FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal VPM19 unconjugated
anti-MHC, Class I antibody适用: 小鼠 IHC, IP, IA 宿主: 兔 Chimeric R1-21-2 unconjugated Recombinant Antibody
anti-MHC, Class I (AA 81-180) antibody适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 小鼠 IP, IF, IA 宿主: 兔 Chimeric R1-9-6 unconjugated Recombinant Antibody
anti-MHC, Class I antibody (PE)适用: 小鸡 FACS, IHC 宿主: 小鼠 Monoclonal F21-2 PE
anti-MHC, Class I antibody适用: 小鸡 FACS, WB, IHC, IP 宿主: 小鼠 Monoclonal F21-2 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: THP-1 and Jurkat cells. Tonsil or lymph node.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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