MECA-79 抗体 (Biotin)
This anti- antibody is a 大鼠 单克隆 antibody detecting in ELISA, FACS, IF, IHC, Func 和 IHC (fp). Suitable for 人, 小鼠 和 大鼠.
Quick Overview for MECA-79 抗体 (Biotin) (ABIN7656727)
抗原
MECA-79
适用
人, 小鼠, 大鼠
宿主
大鼠
克隆类型
单克隆
标记
Biotin
应用范围
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
克隆位点
MECA-79
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原理
- MECA-79(MECA-79), Biotin conjugate
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产品特性
- MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set of antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1, 3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with PNAd from mouse, human and many other species and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Mouse lymph node stromal cells
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亚型
- IgM, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: Human or mouse tonsil.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- MECA-79
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背景
- MECA-79, Peripheral Node Addressin (PNAd)
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分子量
- 90-110 kDa
抗原
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