Maltose Binding Protein 抗体 (CF®594)
Quick Overview for Maltose Binding Protein 抗体 (CF®594) (ABIN7656516)
抗原
See all Maltose Binding Protein (MBP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Maltose Binding Protein / MBP-probe(R29.6), CF594 conjugate
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产品特性
- Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- MOS maltose binding protein fusion protein.
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亚型
- IgG1, kappa
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anti-Maltose Binding Protein (MBP) (AA 43-57) antibody
MBP 适用: E. coli WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Maltose Binding Protein (MBP) antibodyMBP 适用: Bacteria WB, IP 宿主: 小鼠 Monoclonal R29 unconjugated
anti-Maltose Binding Protein (MBP) antibodyMBP 适用: E. coli WB, ELISA 宿主: 小鼠 Monoclonal 10C12 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.
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说明
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Positive Control: Saos-2 cells. MBP fusion proteins.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Maltose Binding Protein (MBP)
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别名
- Maltose Binding Protein
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背景
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Synonyms: ECK4026, JW3994, MalE, malJ, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, Periplasmic maltose binding protein
Gene Symbol: Not Known
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分子量
- 40 kDa
抗原
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