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MHC Class II 抗体 (CF®740)

Name: MHC Class II 抗体 (CF®740)
SKU: ABIN7656453
Availability: OutOfStock

This anti-MHC Class II antibody is a 小鼠单克隆 antibody detecting MHC Class II in . Suitable for 人和猴.

产品编号 ABIN7656453

发货至: 中国

Quick Overview for MHC Class II 抗体 (CF®740) (ABIN7656453)

抗原

See all MHC Class II (MHC2) 抗体

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

适用

人, 猴

宿主

小鼠

克隆类型

单克隆

标记

This MHC Class II antibody is conjugated to CF®740

应用范围

请咨询

克隆位点

IPO-10

Discover our top product MHC2 Primary Antibody

原理

MHC II (HLA-DRA)(IPO-10), CF740 conjugate

产品特性

MAb IPO-10 defines the antigen that appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50 % of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70 % of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

免疫原

Spleen cells of a patient with hairy cell leukemia (Daudi cells)

亚型

IgG3, kappa
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, Func, IHC (p) 宿主: 大鼠 Monoclonal M5-114 unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)
MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC

应用备注

For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

说明

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

限制

仅限研究用
状态

Liquid

浓度

0.1 mg/mL

缓冲液

PBS, 0.1 % rBSA, 0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light

储存条件

4 °C

储存方法

Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light

有效期

24 months
抗原

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

别名

MHC-II

背景

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea
Gene Symbol: HLA-DRA

分子量

36 kDa

基因ID

3122

UniProt

P01903