MHC Class II 抗体 (CF®740)
Quick Overview for MHC Class II 抗体 (CF®740) (ABIN7656451)
抗原
See all MHC Class II (MHC2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MHC II (HLA-DQ)(SPV-L3), CF740 conjugate
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产品特性
- Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- T4-positive CTL clone HG-38
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亚型
- IgG2a, kappa
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anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, Func, IHC (p) 宿主: 大鼠 Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Raji cells. Tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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