MHC Class II 抗体 (CF®740)
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北京 101111
Quick Overview for MHC Class II 抗体 (CF®740) (ABIN7656450)
抗原
See all MHC Class II (MHC2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MHC II (HLA-DP) (beta chain)(BRA-FB6), CF740 conjugate
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产品特性
- Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
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亚型
- IgG2b, kappa
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anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠 FACS, WB, IP, IHC (fro), Func, IHC (p) 宿主: 大鼠 Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Raji cells. Tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
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别名
- MHC-II
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背景
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Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea
Gene Symbol: HLA-DPB1
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分子量
- 33-35 kDa
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基因ID
- 3115
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UniProt
- P04440
抗原
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