Laminin 抗体 (CF®740)
Quick Overview for Laminin 抗体 (CF®740) (ABIN7656313)
抗原
See all Laminin (LN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Laminin(A5), CF740 conjugate
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产品特性
- Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of α, β, and γ chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Murine EHS laminin preparation
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亚型
- IgG2a, kappa
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anti-Laminin (LN) (AA 901-1000) antibody
LN 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 兔, Avian, 小鸡, 犬, 鸭 IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) (AA 31-270) antibodyLN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D9G5 unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, IF, IHC (p), IHC (fro), ICC, Func 宿主: 兔 Polyclonal unconjugated
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: Normal colon or carcinomas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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